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Coding

Part:BBa_K1456002:Design

Designed by: Mustafa Semih Elitok   Group: iGEM14_ATOMS-Turkiye   (2014-07-21)

Glutathione Peroxidase-1 (GPX1)

ATOMS gpx results 2.jpg

Cloning

ATOMS gpx results 5.jpg

  • GPx inserts were acquired from the ordered genes and cDNA. The results showed the expected range of 500-750 bp.


ATOMS gpx results 7.jpg

  • Colony PCR was conducted using the specified primers. As it can be seen on the figure, the results were not accurately fitting the base pair range mentioned. The experiment was repeated several times, and after unsuccessful attempts, the parts were ordered for artificial synthesis.


ATOMS gpx results 9.jpg

  • The ligated and transformated samples were put into colony PCR. As it can be seen on the figure, the right inserts were located in colonies 7,8 and 10.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 31
    Illegal NotI site found at 5
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 89
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 471
    Illegal SapI site found at 346

Design Notes

None


Source

We clonned this part from human hepatocellular carcinoma cell genome cDNA


References